Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia

Rahul Pal; Kert Edward; Tyra Brown; Liang Ma; Jinping Yang; Susan McCammon; Massoud Motamedi; Gracie Vargas

doi:10.1117/12.2005144

22 March 2013 Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia

Rahul Pal, Kert Edward, Tyra Brown, Liang Ma, Jinping Yang, Susan McCammon, Massoud Motamedi, Gracie Vargas

Author Affiliations +

Proceedings Volume 8572, Advanced Biomedical and Clinical Diagnostic Systems XI; 85720M (2013) https://doi.org/10.1117/12.2005144
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Multiphoton Autofluorescence Microscopy (MPAM) and Second Harmonic Generation Microscopy (SHGM) have shown the potential for noninvasive assessment of oral precancers and cancers. We have explored a combination of these nonlinear optical microscopic imaging techniques with widefield fluorescence imaging to assess morphometry similar to that of pathologic evaluation as well as information from endogenous fluorophores, which are altered with neoplastic transformation. Widefield fluorescence revealed areas of interest corresponding to sites with precancers or early tumors, generally resulting in a decrease in green emission or increase in red emission. Subsequent microscopy revealed significant differences in morphology between normal, dysplastic/neoplastic mucosa for all layers. Combination of a widefield and a microscopic technique provides a novel approach for tissue morphometric analysis along with large area assessment of tissue autofluorescence properties.

Citation Download Citation

Rahul Pal, Kert Edward, Tyra Brown, Liang Ma, Jinping Yang, Susan McCammon, Massoud Motamedi, and Gracie Vargas "Combination of widefield fluorescence imaging and nonlinear optical microscopy of oral epithelial neoplasia", Proc. SPIE 8572, Advanced Biomedical and Clinical Diagnostic Systems XI, 85720M (22 March 2013); https://doi.org/10.1117/12.2005144

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