Phase relief imaging with confocal laser scanning system

Tong Peng; Hao Xie; Yichen Ding; Peng Xi

doi:10.1117/12.2001181

22 February 2013 Phase relief imaging with confocal laser scanning system

Tong Peng, Hao Xie, Yichen Ding, Peng Xi

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85871M (2013) https://doi.org/10.1117/12.2001181
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Confocal laser scanning microscopy (CLSM) has become one of the most important biomedical research tools today due to its noninvasive and 3-D abilities. It enables imaging in living tissue with better resolution and contrast, and plays a growing role among microscopic techniques utilized for investigating numerous biological problems. In some cases, the sample was phase-sensitive, thus we introduce a novel method named laser oblique scanning optical microscopy (LOSOM) which could obtain a relief image in transparent sample directly. Through the LOSOM system, mouse kidney and HeLa cells sample were imaged and 10x, 20x and 40x magnify objective imaging results were realized respectively. Also, we compared the variation of pinhole size versus imaging result. One major parameters of LOSOM is the distance between fluorescence medium and the sample. Previously, this distance was set to 1.2 mm, which is the thickness of the slide. The experiment result showed that decreasing d can increase the signal level for LOSOM phase-relief imaging. We have also demonstrated the application of LOSOM in absorption imaging modality, when the specimen is non-transparent.

Citation Download Citation

Tong Peng, Hao Xie, Yichen Ding, and Peng Xi "Phase relief imaging with confocal laser scanning system", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871M (22 February 2013); https://doi.org/10.1117/12.2001181

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Imaging systems

Confocal microscopy

Objectives

Signal detection

Luminescence

Tissues

Absorption

Show All Keywords

Keywords/Phrases

Search In:

Publication Years