DNA aptamer-based fiber optic biosensor for selective and label-free detection of dopamine

M. I. Zibaii; H. Latifi; A. Asadollahi; A. H. Bayat; A. Haghparast

doi:10.1117/12.2193323

28 September 2015 DNA aptamer-based fiber optic biosensor for selective and label-free detection of dopamine

M. I. Zibaii, H. Latifi, A. Asadollahi, A. H. Bayat, A. Haghparast

Proceedings Volume 9634, 24th International Conference on Optical Fibre Sensors; 96340X (2015) https://doi.org/10.1117/12.2193323
Event: International Conference on Optical Fibre Sensors (OFS24), 2015, Curitiba, Brazil

Abstract

Dopamine (DA) analysis is complicated by the interference from other electrochemically active endogenous compounds present in the brain, including DA precursors and metabolites and other neurotransmitters (NT). Here we report a simple, sensitive and selective optical fiber biosensor for the detection of DA in the presence of other NT. It is composed of a 57-mer dopamine-binding aptamer (DBA) as recognition element and nonadiabatic tapered optical fiber (NATOF) as probe. Upon the addition of DA, the conformation of DBA would change from a random coil structure to a rigid tertiary structure like a pocket. The conformational change of DBA lead to the refractive index (RI) change around the tapered fiber surface. Specific recognition of DA by the aptamer allowed a selective optical detection of DA within the physiologically relevant 500 nM to 10 μM range. Some common interferents such as epinephrine (EP) and ascorbic acid (AA) showed no or just a little interference in the determination of DA.

Citation Download Citation

M. I. Zibaii, H. Latifi, A. Asadollahi, A. H. Bayat, and A. Haghparast "DNA aptamer-based fiber optic biosensor for selective and label-free detection of dopamine", Proc. SPIE 9634, 24th International Conference on Optical Fibre Sensors, 96340X (28 September 2015); https://doi.org/10.1117/12.2193323

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