Modelling and implementation of a fixed-length-extension to measure fluorescent intensity in bioprocesses using an optical sensor

Neha Sardesai; Mustafa Al-Adhami; Govind Rao; Yordan Kostov

doi:10.1117/12.2221931

13 May 2016 Modelling and implementation of a fixed-length-extension to measure fluorescent intensity in bioprocesses using an optical sensor

Neha Sardesai, Mustafa Al-Adhami, Govind Rao, Yordan Kostov

Author Affiliations +

Proceedings Volume 9863, Smart Biomedical and Physiological Sensor Technology XIII; 98630J (2016) https://doi.org/10.1117/12.2221931
Event: SPIE Commercial + Scientific Sensing and Imaging, 2016, Baltimore, MD, United States

Abstract

Fluorescent proteins are often used as reporters of protein concentration in biology and biomedicine applications. They can be detected using a fluorimeter equipped with fiber optics for ease of access. However, small changes in the path length due to change in the position, or immersion depth of the optical fiber results in large changes in readings. To alleviate the situation, the fiber is equipped with a fixed-length-extension that provides constant path length. The operation of the fiber equipped fluorimeter is theoretically modelled and practically verified in this paper.

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Neha Sardesai, Mustafa Al-Adhami, Govind Rao, and Yordan Kostov "Modelling and implementation of a fixed-length-extension to measure fluorescent intensity in bioprocesses using an optical sensor", Proc. SPIE 9863, Smart Biomedical and Physiological Sensor Technology XIII, 98630J (13 May 2016); https://doi.org/10.1117/12.2221931

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