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We developed a Raman scattering microscope using a 0th-order Bessel beam to illuminate the inside of a biological sample. We constructed a Raman spectroscopic system in which the Bessel beam illuminates the sample from the side, and the illuminated area in the sample is imaged on the slit of a spectrophotometer. By using the developed microscope, polymer beads and biological samples were observed to confirm the imaging property and the improvement of the image contrast compared to the conventional line-illumination Raman microscopy.
Katsumasa Fujita andKota Koike
"Slit-scanning Raman microscopy using Bessel beam illumination", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730B (2 March 2022); https://doi.org/10.1117/12.2609733
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Katsumasa Fujita, Kota Koike, "Slit-scanning Raman microscopy using Bessel beam illumination," Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730B (2 March 2022); https://doi.org/10.1117/12.2609733