CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC11973 > Article
Presentation
2 March 2022 Transient absorption imaging of mitochondrial redox in muscle fibers
Erkang Wang, Jesse Wilson, Adam J Chicco, Luke A whitcomb
Author Affiliations +
Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730L (2022) https://doi.org/10.1117/12.2608844
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
We present label-free transient absorption microscopy (TAM) of mitochondrial redox in live muscle fibers. Image stacks consisting of TAM images at different pump-probe delays, processed with principal component analysis (PCA) reveal picosecond timescale differences between reduced and oxidized mitochondria, in addition to heterogeneity among mitochondria within individual muscle fibers. We will discuss these findings in light of the photoinduced transient conformational and vibronic states of mitochondrial respiratory chain hemeproteins and relaxation pathways.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Erkang Wang, Jesse Wilson, Adam J Chicco, and Luke A whitcomb "Transient absorption imaging of mitochondrial redox in muscle fibers", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730L (2 March 2022); https://doi.org/10.1117/12.2608844
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Absorption
Optical fibers
Molecules
Principal component analysis
In vivo imaging
Microscopy
Multiphoton microscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top