Single particle observations of CRISPR nanoplasmonic carriers

Zhijia Zhang; Haechan Jeong; Di Zu; Xintao Zhao; Guangjie Cui; Somin Eunice Lee

doi:10.1117/12.2610222

3 March 2022 Single particle observations of CRISPR nanoplasmonic carriers

Zhijia Zhang, Haechan Jeong, Di Zu, Xintao Zhao, Guangjie Cui, Somin Eunice Lee

Proceedings Volume PC11978, Plasmonics in Biology and Medicine XIX; PC1197804 (2022) https://doi.org/10.1117/12.2610222
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

The method of delivery is considered to be one of the significant hurdles for CRISPR technologies. Conventional delivery of CRISPR by viral vectors are subject to their immunogenicity. Non-viral vectors methods are preferable but are limited by their biocompatibility and precision. Nano-plasmonic particles have shown great potential as noninvasive and spatiotemporally controllable vehicles for biomacromolecule delivery. Hereby, as a safe and precise way to delivery of CRISPR, we propose a remote optically manipulating method based on gold nanorods carrier. We have characterized gold nano-plasmonic carriers on single particle level by imaging spectroscopy. Then we have demonstrated the precise manipulation of individual nano plasmonic carriers on cells using optical trapping system. Lastly, the biocompatibility of the method is shown.

Conference Presentation

Citation Download Citation

Zhijia Zhang, Haechan Jeong, Di Zu, Xintao Zhao, Guangjie Cui, and Somin Eunice Lee "Single particle observations of CRISPR nanoplasmonic carriers", Proc. SPIE PC11978, Plasmonics in Biology and Medicine XIX, PC1197804 (3 March 2022); https://doi.org/10.1117/12.2610222

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