Albert Claude Boccara,1 Viacheslav Mazlin,2 Olivier A Thouvenin,2 Samer Alhaddad,1 Martine Boccara3
1Institut Langevin (France) 2Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris, Univ. PSL (France) 3Ecole Normale Supérieure, Univ. PSL (France)
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We have recently (BOE July 2022) proposed an interferometric approach called full-field transmission tomography (FFOTT) based on the use of the Gouy phase shift that manifests at the focus of microscope objectives. Forward scattering of cellular structures larger than 100 nm being much greater than backscattering (used in OCT) performances constraints of the imaging system are strongly relaxed and setups using cheap microscopes and a smartphones become possible. Note that good quality 100X, NA+1.25 objectives are available for less than $100.
We show cells and tissues images through their morphological or metabolic contrasts modified by a chemical or biological environments.
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Albert Claude Boccara, Viacheslav Mazlin, Olivier A Thouvenin, Samer Alhaddad, Martine Boccara, "Static and dynamic full field optical transmission tomography in the 100/500 dollar range," Proc. SPIE PC12369, Optics and Biophotonics in Low-Resource Settings IX, PC1236902 (6 March 2023); https://doi.org/10.1117/12.2648433