Computational multiphoton microscopy for bi-functional fluorescence and refractive index imaging (Conference Presentation)

Yi Xue

doi:10.1117/12.2649459

15 March 2023 Computational multiphoton microscopy for bi-functional fluorescence and refractive index imaging (Conference Presentation)

Yi Xue

Author Affiliations +

Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840B (2023) https://doi.org/10.1117/12.2649459
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Multimodal imaging with optical microscopy that can image both fluorescence-labeled and label-free structures in the same sample enables correlating the two. We previously developed one-photon bi-functional microscopy that can reconstruct 3D fluorescence and 3D refractive index from the same fluorescence images captured in epi-mode, but this technique only works for samples consisting of sparsely labeled fluorescence structures and weak scattering media. Our new work jointly develops multiphoton microscopy and a physics-based computational model for simultaneously imaging fluorescence and refractive index in strong scattering samples.

Conference Presentation

Citation Download Citation

Yi Xue "Computational multiphoton microscopy for bi-functional fluorescence and refractive index imaging (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840B (15 March 2023); https://doi.org/10.1117/12.2649459

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