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For patients suffering from cancer or neurodegenerative diseases research into the underlying causes is of the highest importance. Here, we report on our efforts to optimize higher harmonic generation microscopy complemented with multi-photon (auto)fluorescence, to image for long period of times on acute human tissue slices. Results for time-lapse imaging of the induction of myelin-swelling in Multiple Sclerosis brains during gradual high sodium perfusion and fibrosis induced in samples obtained from lobectomy, in the context of pulmonary fibrosis will be presented.
Marie L. Groot,Frank van Mourik,Niels Meijns,Yuanyuan Ma, andOliver Prochnow
"Optimization of higher harmonic generation microscopy for acute tissue imaging (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840X (15 March 2023); https://doi.org/10.1117/12.2668349
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Marie L. Groot, Frank van Mourik, Niels Meijns, Yuanyuan Ma, Oliver Prochnow, "Optimization of higher harmonic generation microscopy for acute tissue imaging (Conference Presentation)," Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840X (15 March 2023); https://doi.org/10.1117/12.2668349