Quantitative phase microscopy for life science and diagnosis (Conference Presentation)

Balpreet Singh Ahluwalia; Azeem Ahmad; Vishesh Kumar Dubey; Ankit Butola

doi:10.1117/12.2659335

16 March 2023 Quantitative phase microscopy for life science and diagnosis (Conference Presentation)

Balpreet Singh Ahluwalia, Azeem Ahmad, Vishesh Kumar Dubey, Ankit Butola

Proceedings Volume PC12389, Quantitative Phase Imaging IX; PC123890G (2023) https://doi.org/10.1117/12.2659335
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We report high-speed and highly sensitive quantitative phase microscopy (QPM) using dynamic speckle illumination (DSI). The DSI-QPM is used for real-time analyses of highly motile human spermatozoa. The DSI-QPM supports high-speed and high spatial phase sensitivity, that are crucial for imaging tail (nanoscale) of living spermatozoa during motion. The scalable FoV and high temporal coherence offered by DSI-QPM is harnessed for histopathology and marine biology. Further, by integrating the single molecule localization microscopy (SMLM) with QPM, nanoscale imaging and quantification in lateral (via SMLM) and axial (via QPM) directions was achieved on liver cells.

Conference Presentation

Citation Download Citation

Balpreet Singh Ahluwalia, Azeem Ahmad, Vishesh Kumar Dubey, and Ankit Butola "Quantitative phase microscopy for life science and diagnosis (Conference Presentation)", Proc. SPIE PC12389, Quantitative Phase Imaging IX, PC123890G (16 March 2023); https://doi.org/10.1117/12.2659335

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