Super resolution multimodal metabolic imaging in aging and diseases (Conference Presentation)

Lingyan Shi; Yajuan Li; Hongje Jang; Zhi Li; Sahran Hussain

doi:10.1117/12.2648000

15 March 2023 Super resolution multimodal metabolic imaging in aging and diseases (Conference Presentation)

Lingyan Shi, Yajuan Li, Hongje Jang, Zhi Li, Sahran Hussain

Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920A (2023) https://doi.org/10.1117/12.2648000
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We use D2O probed stimulated Raman scattering (DO-SRS) and multiphoton fluorescence (MPF) microscopy to visualize metabolic changes in cells and tissues during aging processes and diseases. We further developed deconvolution algorithm to enhance the spatial resolution to generate super resolution metabolic imaging. The cellular spatial distribution of de novo lipogenesis, protein synthesis, NADH, Flavin, unsaturated lipids, and cholesterol were all imaged and quantified with hyperspectral detection of various macromolecules as multiplex imaging. Our study shows that the integrated metabolic imaging and spectroscopy platform can be applied to study nutrition regulated metabolic dynamics in cancer and during aging processes.

Conference Presentation

Citation Download Citation

Lingyan Shi, Yajuan Li, Hongje Jang, Zhi Li, and Sahran Hussain "Super resolution multimodal metabolic imaging in aging and diseases (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920A (15 March 2023); https://doi.org/10.1117/12.2648000

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available