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15 March 2023 Label-free parts per million detection by autofluorescence photothermal mid-IR (AF-PTIR) microscopy (Conference Presentation)
Aleksandr Razumtcev, Minghe Li, Garth J. Simpson
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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920W (2023) https://doi.org/10.1117/12.2649064
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
AF-PTIR microscopy is shown to enable chemically-specific imaging of an active pharmaceutical ingredient (API) and discrimination between two crystal forms of the API with parts per million (ppm) detection limit. AF-PTIR is based on detecting highly-localized temperature-induced variations in native autofluorescence intensity following the absorption of mid-IR radiation generated by an array of 32 independent narrowband quantum cascade lasers (QCLs). AF-PTIR was demonstrated to improve the detection limit in trace crystal form impurities analysis by two orders of magnitude as compared to commercial powder X-ray diffraction instrumentation, suggesting potential applications in pharmaceutical formulations analysis.
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Aleksandr Razumtcev, Minghe Li, and Garth J. Simpson "Label-free parts per million detection by autofluorescence photothermal mid-IR (AF-PTIR) microscopy (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920W (15 March 2023); https://doi.org/10.1117/12.2649064
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KEYWORDS
Mid-IR
Crystals
Microscopy
Absorption
Imaging spectroscopy
Quantum cascade lasers
Solids
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