High-speed Raman spectroscopy has enabled high-dimensional phenotyping of cells. Among these, time-domain Raman spectroscopy, such as Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS), has unique capabilities such as high spectral acquisition rate, broadband spectral sensitivity in the fingerprint region, and nonresonant-background-free spectral acquisition. In this talk, we will present our recent achievements in FT-CARS spectroscopy for life science, including super-multiplex flow cytometry, high-speed dual-band spectroscopy, and broadband Raman-activated cell sorting.
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