Plasmonic solid-state nanopores: toward single-molecule protein and DNA identification

Francesco De Angelis

doi:10.1117/12.2653096

17 March 2023 Plasmonic solid-state nanopores: toward single-molecule protein and DNA identification

Francesco De Angelis

Proceedings Volume PC12425, Smart Photonic and Optoelectronic Integrated Circuits 2023; PC124250M (2023) https://doi.org/10.1117/12.2653096
Event: SPIE OPTO, 2023, San Francisco, California, United States

Abstract

The success of nanopore sequencers for DNA has inspired extensive research on proteins. However, when moving to proteins some major challenges remain, among them: 1) DNA bases are just 4 against the amino acids which are 20; (2) spatial and temporal resolution (sensitivity) to discriminate single amino acids within the same molecule. In this context, label-free optical analysis based on plasmonic enhancement shows great promises. In fact, plasmonic nanopores can confine and amplifying the local electromagnetic field into the pore (challenge #2). The confinement improves the spatial resolution while the amplification increases the sensitivity. Notably, Raman spectroscopy provides unique molecular fingerprints to discriminate amino acids (challenge #1). We show our latest results on extreme plasmonic nanopores combined with Raman Spectroscopy for amino acids identification and sequencing at single molecule level in label-free. We acknowledge support from Horizon 2020 (ProID GA 964363).

Conference Presentation

Citation Download Citation

Francesco De Angelis "Plasmonic solid-state nanopores: toward single-molecule protein and DNA identification", Proc. SPIE PC12425, Smart Photonic and Optoelectronic Integrated Circuits 2023, PC124250M (17 March 2023); https://doi.org/10.1117/12.2653096

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available