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5 October 2023 Study and regulation of biomolecular interactions at electrified interfaces
Sasha A. Moonitz, McKenzie Jonely, Rodrigo Noriega Manez
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Proceedings Volume PC12650, Physical Chemistry of Semiconductor Materials and Interfaces XXII; PC1265005 (2023) https://doi.org/10.1117/12.2676816
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States
Abstract
Our efforts to disentangle the link between molecular recognition and biochemical function are centered on the study of binding and dynamics of protein-RNA complexes at electrified interfaces. We are interested in the effects of electrostatics and local dynamics on the formation and stability of RNA-protein complexes. The use of complementary multimodal observations enables us to distinguish between distinct stages in the binding event between an RNA-binding protein and its target -- both of which are affected by interfacial electric fields. To follow dynamic events over multiple time scales, we combine mid-infrared surface plasmonics, time-resolved ultrafast fluorescence, and in situ electrochemical experiments at the surface of a degenerately doped wide-gap metal oxide.
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Sasha A. Moonitz, McKenzie Jonely, and Rodrigo Noriega Manez "Study and regulation of biomolecular interactions at electrified interfaces", Proc. SPIE PC12650, Physical Chemistry of Semiconductor Materials and Interfaces XXII, PC1265005 (5 October 2023); https://doi.org/10.1117/12.2676816
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KEYWORDS
Molecular interactions
Proteins
Electric fields
Interfaces
Biomolecules
Mid-IR
Plasmonics
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