Metabolic FLIM and oxygen PLIM/dFLIM in translational research

Angelika C. Rueck; Johannes Wieland; Kirsten Reess; Daniela dos Santos; Nilanjon Naskar

doi:10.1117/12.3001595

13 March 2024 Metabolic FLIM and oxygen PLIM/dFLIM in translational research

Angelika C. Rueck, Johannes Wieland, Kirsten Reess, Daniela dos Santos, Nilanjon Naskar

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470P (2024) https://doi.org/10.1117/12.3001595
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Fluorescence lifetime imaging (FLIM) of metabolic coenzymes, as NAD(P)H and FAD and oxygen induced phosphorescence lifetime imaging (PLIM) of phosphorescent drugs or delayed FLIM (dFLIM) is now widely accepted to be one of the most important imaging methods for cell metabolism and oxygen. Different algorithms are developed and optimized for simultaneous FLIM/PLIM/dFLIM to get reproducible results. The interaction of light, drug and oxygen during PDT (photodynamic therapy) induces a cellular response, which could be followed by various techniques. The aim of this work was to simultaneously image the cellular response and oxygen consumption in living cells with subcellular resolution.

Conference Presentation

Citation Download Citation

Angelika C. Rueck, Johannes Wieland, Kirsten Reess, Daniela dos Santos, and Nilanjon Naskar "Metabolic FLIM and oxygen PLIM/dFLIM in translational research", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470P (13 March 2024); https://doi.org/10.1117/12.3001595

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