Front Matter

Table of Contents

• Front Matter

• Part I. Optical Microscopy
• 1. A Brief History of the Microscope and Its Significance in the Advancement of Biology and Medicine

• 2. The Optical Microscope: Its Principles, Components, and Limitations

• 3. Abbe Theory of Image Formation and Diffraction of Light in Transmitted Light Microscopes

• 4. Optical Resolution and Resolving Power: What It Is, How to Measure It, and What Limits It

• 5. Techniques That Provide Contrast

• Part II. Confocal Microscopy
• 6. Early Antecedents of Confocal Microscopy

• 7. Optical Sectioning (Depth Discrimination) with Different Scanning Techniques: The Beginnings of Confocal Microscopy

• 8. The Development of Scanning-Slit Confocal Systems for Imaging Live Cells, Tissues, and Organs

• 9. The Components of a Confocal Microscope

• Part III. Nonlinear Microscopy
• 10. The Development of Nonlinear Spectroscopy and Microscopy

• 11. Multiphoton Excitation Microscopy

• 12. Theory and Instrumentation of Multiphoton Excitation Microscopy

• Part IV. The Path to Imaging Live Cells, Tissues, and Organs
• 13. Remaining Problems, Limitations, and Their Partial Solutions

• 14. Speculation on Future Directions for Confocal and Multiphoton Excitation Microscopy

• 15. Safety and Cleanliness Considerations

• Back Matter