Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source

Yokoyama, Hiroyuki; Tsubokawa, Hiroshi; Guo, Hengchang; Shikata, Jun-ichi; Sato, Ki-ichi; Takashima, Keijiro; Kashiwagi, Kaori; Saito, Naoaki; Taniguchi, Hirokazu; Ito, Hiromasa

doi:doi:10.1117/1.2800393

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Journal of Biomedical Optics / Volume 12 / Issue 5 / Research Papers

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Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source

J. Biomed. Opt. 12, 054019 (Nov 01, 2007); http://dx.doi.org/10.1117/1.2800393

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Abstract

References (25)

Citing Articles (7)

Hiroyuki Yokoyama, Hengchang Guo, Ki-ichi Sato, and Keijiro Takashima

Tohoku University, New Industry Creation Hatchery Center (NICHe), 6-6-10 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan

Hiroshi Tsubokawa

Tohoku University, Graduate School of Information Sciences, 6-3-09 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan

Jun-ichi Shikata, Hirokazu Taniguchi, and Hiromasa Ito

Tohoku University, Research Institute of Electrical Communication (RIEC), 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan

Kaori Kashiwagi and Naoaki Saito

Kobe University, Biosignal Research Center, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan

We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).

History

Received May 25, 2007
Accepted Aug 23, 2007
Revised Aug 22, 2007
Published online Nov 01, 2007

Digital Object Identifier

http://dx.doi.org/10.1117/1.2800393

Citation

Hiroyuki Yokoyama, Hiroshi Tsubokawa, Hengchang Guo, Jun-ichi Shikata, Ki-ichi Sato, Keijiro Takashima, Kaori Kashiwagi, Naoaki Saito, Hirokazu Taniguchi and Hiromasa Ito, "Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source", J. Biomed. Opt. 12, 054019 (Nov 01, 2007); http://dx.doi.org/10.1117/1.2800393