Monitoring of hexyl 5-aminolevulinate-induced photodynamic therapy in rat bladder cancer by optical spectroscopy

Larsen, Eivind L. P.; Randeberg, Lise L.; Gederaas, Odrun A.; Arum, Carl-Jørgen; Hjelde, Astrid; Zhao, Chun-Mei; Chen, Duan; Krokan, Hans E.; Svaasand, Lars O.

doi:doi:10.1117/1.2967909

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Journal of Biomedical Optics / Volume 13 / Issue 4 / Research Papers

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Monitoring of hexyl 5-aminolevulinate-induced photodynamic therapy in rat bladder cancer by optical spectroscopy

J. Biomed. Opt. 13, 044031 (Aug 26, 2008); http://dx.doi.org/10.1117/1.2967909

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Abstract

References (36)

Citing Articles (5)

Eivind L. P. Larsen, Lise L. Randeberg, and Lars O. Svaasand

Norwegian University of Science and Technology, Department of Electronics and Telecommunications, N-7034 Trondheim, Norway

Odrun A. Gederaas, Chun-Mei Zhao, Duan Chen, and Hans E. Krokan

Norwegian University of Science and Technology, Department of Cancer Research and Molecular Medicine, N-7006 Trondheim, Norway

Carl-Jørgen Arum

Norwegian University of Science and Technology, Department of Cancer Research and Molecular Medicine, N-7006 Trondheim, Norway and St. Olavs Hospital, Trondheim University Hospital, Department of Surgery, N-7006 Trondheim, Norway

Astrid Hjelde

Norwegian University of Science and Technology, Department of Circulation and Medical Imaging, N-7006 Trondheim, Norway

Monitoring of the tissue response to photodynamic therapy (PDT) can provide important information to help optimize treatment variables such as drug and light dose, and possibly predict treatment outcome. A urinary bladder cancer cell line (AY-27) was used to induce orthotopic transitional cell carcinomas (TCC) in female Fischer rats, and hexyl 5-aminolevulinate (HAL, 8 mM, 1 h)-induced PDT was performed on day 14 after instillation of the cancer cells (20 J/cm² fluence at 635 nm). In vivo optical reflectance and fluorescence spectra were recorded from bladders before and after laser treatment with a fiberoptic probe. Calculated fluorescence bleaching and oxygen saturation in the bladder wall were examined and correlated to histology results. Reflectance spectra were analyzed using a three-layer optical photon transport model. Animals with TCC treated with PDT showed a clear treatment response; decreased tissue oxygenation and protoporphyrin IX (PpIX) fluorescence photobleaching were observed. Histology demonstrated that 3 of 6 animals with treatment had no sign of the tumor 7 days after PDT treatment. The other 3 animals had significantly reduced the tumor size. The most treatment-responsive animals had the highest PpIX fluorescence prior to light irradiation. Thus, optical spectroscopy can provide useful information for PDT. The model has proved to be very suitable for bladder cancer studies.

History

Received Dec 17, 2007
Accepted Mar 25, 2008
Revised Mar 19, 2008
Published online Aug 26, 2008

Digital Object Identifier

http://dx.doi.org/10.1117/1.2967909

Citation

Eivind L. P. Larsen, Lise L. Randeberg, Odrun A. Gederaas, Carl-Jørgen Arum, Astrid Hjelde, Chun-Mei Zhao, Duan Chen, Hans E. Krokan and Lars O. Svaasand, "Monitoring of hexyl 5-aminolevulinate-induced photodynamic therapy in rat bladder cancer by optical spectroscopy", J. Biomed. Opt. 13, 044031 (Aug 26, 2008); http://dx.doi.org/10.1117/1.2967909