In vivo Raman flow cytometry for real-time detection of carbon nanotube kinetics in lymph, blood, and tissues

Biris, Alexandru S.; Galanzha, Ekaterina I.; Li, Zhongrui; Mahmood, Meena; Xu, Yang; Zharov, Vladimir P.

doi:doi:10.1117/1.3119145

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Journal of Biomedical Optics / Volume 14 / Issue 2 / Special Section on Nanophotonics for Diagnostics, Protection, and Treatment of Cancer and Inflammatory Diseases

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In vivo Raman flow cytometry for real-time detection of carbon nanotube kinetics in lymph, blood, and tissues

J. Biomed. Opt. 14, 021006 (Apr 28, 2009); http://dx.doi.org/10.1117/1.3119145

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Abstract

References (28)

Citing Articles (14)

Alexandru S. Biris, Zhongrui Li, Meena Mahmood, and Yang Xu

University of Arkansas at Little Rock, Applied Science Department, Nanotechnology Center, Little Rock, Arkansas 72204

Ekaterina I. Galanzha and Vladimir P. Zharov

University of Arkansas for Medical Sciences, Philips Classic Laser and Nanomedicine Laboratories, Little Rock, Arkansas 72205

Nanoparticles are intensively being explored as contrast agents for medical diagnostics and therapies using various optical methods. We present the first demonstration of the use of time-resolved Raman spectroscopy for in vivo real-time detection of circulating carbon nanotubes (CNTs) or cancer cells labeled with CNTs in the lymph, blood, and tissues of live animals with fast spectral acquisition times of down to few milliseconds. After intravenously administering CNTs in the tail vein of the rat, this technique provides the ability to detect the circulation of CNTs in the blood microvessels of the intact rat ear. The capability of Raman spectroscopy is also demonstrated to monitor, identify, and image the CNTs during their transportation by lymphatics in the rat ear and mesentery. The strong and specific Raman scattering properties of CNTs make it possible to detect in vitro and in vivo single cancer cells (HeLa) tagged with CNTs. In vivo Raman flow cytometry opens a new avenue for multiparameter analysis of circulating nanoparticles with strong Raman scattering properties and their pharmokinetics in blood and lymph systems. Moreover, this technology has the potential for molecular detection and identification of circulating tumor cells, and infections labeled with CNTs.

History

Received Aug 08, 2008
Accepted Feb 15, 2009
Revised Feb 11, 2009
Published online Apr 28, 2009

Digital Object Identifier

http://dx.doi.org/10.1117/1.3119145

Citation

Alexandru S. Biris, Ekaterina I. Galanzha, Zhongrui Li, Meena Mahmood, Yang Xu and Vladimir P. Zharov, "In vivo Raman flow cytometry for real-time detection of carbon nanotube kinetics in lymph, blood, and tissues", J. Biomed. Opt. 14, 021006 (Apr 28, 2009); http://dx.doi.org/10.1117/1.3119145