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Journal of Biomedical Optics / Volume 16 / Issue 12 / JBO Letters

Time-resolved imaging refractometry of microbicidal films using quantitative phase microscopy

J. Biomed. Opt. 16, 120510 (Dec 19, 2011); http://dx.doi.org/10.1117/1.3665439

Matthew T. Rinehart, Tyler K. Drake, Francisco E. Robles, and Adam Wax

Duke University, Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, 3000 Science Drive, 136 Hudson Hall, Box 90281, Durham, North Carolina 27708

Lisa C. Rohan

University of Pittsburgh, School of Pharmacy, Magee Womens Research Institute, Pittsburgh, Pennsylvania 15213

David Katz

Duke University, Department of Biomedical Engineering, Center for Biomolecular and Tissue Engineering, 136 Hudson Hall, Box 90281, Durham, North Carolina 27708

Quantitative phase microscopy is applied to image temporal changes in the refractive index (RI) distributions of solutions created by microbicidal films undergoing hydration. We present a novel method of using an engineered polydimethylsiloxane structure as a static phase reference to facilitate calibration of the absolute RI across the entire field. We present a study of dynamic structural changes in microbicidal films during hydration and subsequent dissolution. With assumptions about the smoothness of the phase changes induced by these films, we calculate absolute changes in the percentage of film in regions across the field of view.

© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

History
Received Sep 26, 2011
Accepted Nov 14, 2011
Revised Nov 09, 2011
Published online Dec 19, 2011
Digital Object Identifier
http://dx.doi.org/10.1117/1.3665439
Citation
Matthew T. Rinehart, Tyler K. Drake, Francisco E. Robles, Lisa C. Rohan, David Katz and Adam Wax, "Time-resolved imaging refractometry of microbicidal films using quantitative phase microscopy", J. Biomed. Opt. 16, 120510 (Dec 19, 2011); http://dx.doi.org/10.1117/1.3665439

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