Lensless multispectral digital in-line holographic microscope

Ryle, James P.; McDonnell, Susan; Sheridan, John T.

doi:doi:10.1117/1.3659681

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Journal of Biomedical Optics / Volume 16 / Issue 12 / Research Papers: Imaging

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Lensless multispectral digital in-line holographic microscope

J. Biomed. Opt. 16, 126004 (Dec 16, 2011); http://dx.doi.org/10.1117/1.3659681

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Abstract

References (71)

James P. Ryle and John T. Sheridan

University College Dublin, Communications and Optoelectronic Research Centre, Belfield, Dublin D4, Ireland

University College Dublin, SFI Strategic Research Cluster in Solar Energy Conversion, Belfield, Dublin D4, Ireland

University College Dublin, School of Electrical, Electronic and Communications Engineering, Belfield, Dublin D4, Ireland

Susan McDonnell

University College Dublin, School of Chemical and Bioprocess Engineering, College of Engineering, Mathematical, and Physical Sciences, Belfield, Dublin D4, Ireland

An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.

History

Received Jun 08, 2011
Accepted Oct 20, 2011
Revised Sep 21, 2011
Published online Dec 16, 2011

Digital Object Identifier

http://dx.doi.org/10.1117/1.3659681

Citation

James P. Ryle, Susan McDonnell and John T. Sheridan, "Lensless multispectral digital in-line holographic microscope", J. Biomed. Opt. 16, 126004 (Dec 16, 2011); http://dx.doi.org/10.1117/1.3659681