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Lensless multispectral digital in-line holographic microscope
J. Biomed. Opt. 16, 126004 (Dec 16, 2011); http://dx.doi.org/10.1117/1.3659681
An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)
History
Received Jun 08, 2011
Accepted Oct 20, 2011
Revised Sep 21, 2011
Published online Dec 16, 2011
Accepted Oct 20, 2011
Revised Sep 21, 2011
Published online Dec 16, 2011
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Citation
James P. Ryle, Susan McDonnell and John T. Sheridan, "Lensless multispectral digital in-line holographic microscope",
J. Biomed. Opt. 16, 126004 (Dec 16, 2011); http://dx.doi.org/10.1117/1.3659681
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