Long-distance fluorescence lifetime imaging using stimulated emission

Dellwig, Thilo; Lin, Po-Yen; Kao, Fu-Jen

doi:doi:10.1117/1.JBO.17.1.011009

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Journal of Biomedical Optics / Volume 17 / Issue 1 / Special Section on FRET at 65

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Long-distance fluorescence lifetime imaging using stimulated emission

J. Biomed. Opt. 17, 011009 (Feb 06, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.011009

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Abstract

References (36)

Thilo Dellwig, Po-Yen Lin, and Fu-Jen Kao

National Yang-Ming University, Institute of Biophotonics, Taipei 11221, Taiwan

Long-distance stimulated emission imaging has recently been demonstrated as a novel approach for the characterization and imaging of samples containing fluorescent moieties. We present an extension of this methodology through a pump-probe setup for fluorescence lifetime determination and imaging. We measure fluorescence lifetimes of Rhodamine 6G at different solutions and indocyanine green using long-distance fluorescence lifetime imaging.

History

Received Jun 02, 2011
Accepted Oct 11, 2011
Revised Oct 08, 2011
Published online Feb 06, 2012

Digital Object Identifier

http://dx.doi.org/10.1117/1.JBO.17.1.011009

Citation

Thilo Dellwig, Po-Yen Lin and Fu-Jen Kao, "Long-distance fluorescence lifetime imaging using stimulated emission", J. Biomed. Opt. 17, 011009 (Feb 06, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.011009