High-throughput optofluidic system for the laser microsurgery of oocytes

Chandsawangbhuwana, Charlie; Shi, Linda Z.; Zhu, Qingyuan; Alliegro, Mark C.; Berns, Michael W.

doi:doi:10.1117/1.JBO.17.1.015001

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Journal of Biomedical Optics / Volume 17 / Issue 1 / Research Papers: General

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High-throughput optofluidic system for the laser microsurgery of oocytes

J. Biomed. Opt. 17, 015001 (Feb 07, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.015001

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Abstract

References (23)

Charlie Chandsawangbhuwana, Linda Z. Shi, Qingyuan Zhu, and Michael W. Berns

University of California, San Diego, Department of Bioengineering, 9500 Gilman Drive, La Jolla, California 92093

Mark C. Alliegro

Bay Paul Center for Comparative Molecular Biology, Marine Biological Laboratory, Woods Hole, Massachusetts 02543

University of California, Irvine, Beckman Laser Institute, 1002 Health Sciences Road, Irvine, California 92612

This study combines microfluidics with optical microablation in a microscopy system that allows for high-throughput manipulation of oocytes, automated media exchange, and long-term oocyte observation. The microfluidic component of the system transports oocytes from an inlet port into multiple flow channels. Within each channel, oocytes are confined against a microfluidic barrier using a steady fluid flow provided by an external computer-controlled syringe pump. This allows for easy media replacement without disturbing the oocyte location. The microfluidic and optical-laser microbeam ablation capabilities of the system were validated using surf clam (Spisula solidissima) oocytes that were immobilized in order to permit ablation of the 5 μm diameter nucleolinus within the oocyte nucleolus. Oocytes were the followed and assayed for polar body ejection.

History

Received Aug 16, 2011
Accepted Nov 02, 2011
Revised Nov 02, 2011
Published online Feb 07, 2012

Digital Object Identifier

http://dx.doi.org/10.1117/1.JBO.17.1.015001

Citation

Charlie Chandsawangbhuwana, Linda Z. Shi, Qingyuan Zhu, Mark C. Alliegro and Michael W. Berns, "High-throughput optofluidic system for the laser microsurgery of oocytes", J. Biomed. Opt. 17, 015001 (Feb 07, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.015001