Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy

Huang, Deqiu; Zhang, Wen; Zhong, Huiqing; Xiong, Honglian; Guo, Xi; Guo, Zhouyi

doi:doi:10.1117/1.JBO.17.1.015004

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Journal of Biomedical Optics / Volume 17 / Issue 1 / Research Papers: General

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Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy

J. Biomed. Opt. 17, 015004 (Feb 06, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.015004

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Abstract

References (54)

Deqiu Huang, Wen Zhang, Huiqing Zhong, Honglian Xiong, Xi Guo, and Zhouyi Guo

South China Normal University, Laboratory of Photonic Chinese Medicine, MOE Key Laboratory of Laser Life Science, College of Biophotonics, 510631, Guangzhou, Guangdong, China

In present work, we studied the effect of optical clearing on porcine skin in vitro with glycerol by Raman microspectroscopy, denoted as RM, at various time intervals of 0, 15, 30, 45, 60, and 75 min respectively. The results showed that the addition of glycerol significantly improved the depth of RM measurement, and enhanced the recovery of skin tissue Raman spectra that were not overlapped with the glycerol Raman spectra over time. Moreover, it was found that the Raman signals resembled the native spectrum of the molecules in porcine skin with a negligible frequency shift. Furthermore, we evaluated the extent of optical clearing in porcine skin by utilizing various concentrations of 40%, 60%, and 80% glycerol solution. The results demonstrated that with the increase of concentration of glycerol, the optical clearing of porcine skin was much improved.

History

Received Aug 27, 2011
Accepted Nov 08, 2011
Revised Oct 26, 2011
Published online Feb 06, 2012

Digital Object Identifier

http://dx.doi.org/10.1117/1.JBO.17.1.015004

Citation

Deqiu Huang, Wen Zhang, Huiqing Zhong, Honglian Xiong, Xi Guo and Zhouyi Guo, "Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy", J. Biomed. Opt. 17, 015004 (Feb 06, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.015004