Tryptophan autofluorescence imaging of neoplasms of the human colon

Banerjee, Bhaskar; Renkoski, Timothy; Graves, Logan R.; Rial, Nathaniel S.; Tsikitis, Vassiliki Liana; Nfonsam, Valentine; Pugh, Judith; Tiwari, Piyush; Gavini, Hemanth; Utzinger, Urs

doi:doi:10.1117/1.JBO.17.1.016003

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Journal of Biomedical Optics / Volume 17 / Issue 1 / Research Papers: Imaging

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Tryptophan autofluorescence imaging of neoplasms of the human colon

J. Biomed. Opt. 17, 016003 (Feb 01, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.016003

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Abstract

References (52)

Bhaskar Banerjee, Nathaniel S. Rial, Piyush Tiwari, and Hemanth Gavini

University of Arizona, Department of Medicine, Section of Gastroenterology, 1501 N. Campbell Avenue, PO Box 245028, Tucson, Arizona 85724-5028

Urs Utzinger

University of Arizona, Department of Biomedical Engineering, 1501 N. Campbell Avenue, PO Box 245028, Tucson, Arizona 85724-5028

Timothy Renkoski, Logan R. Graves,

University of Arizona, College of Optical Sciences, 1501 N. Campbell Avenue, PO Box 245028, Tucson, Arizona 85724-5028

Vassiliki Liana Tsikitis and Valentine Nfonsam

University of Arizona, Department of Surgery, 1501 N. Campbell Avenue, PO Box 245028, Tucson, Arizona 85724-5028

Judith Pugh

University of Arizona, Department of Pathology, 1501 N. Campbell Avenue, PO Box 245028, Tucson, Arizona 85724-5028

Detection of flat neoplasia is a major challenge in colorectal cancer screening, as missed lesions can lead to the development of an unexpected ‘incident’ cancer prior to the subsequent endoscopy. The use of a tryptophan-related autofluorescence has been reported to be increased in murine intestinal dysplasia. The emission spectra of cells isolated from human adenocarcinoma and normal mucosa of the colon were studied and showed markedly greater emission intensity from cancerous cells compared to cells obtained from the surrounding normal mucosa. A proto-type multispectral imaging system optimized for ultraviolet macroscopic imaging of tissue was used to obtain autofluorescence images of surgical specimens of colonic neoplasms and normal mucosa after resection. Fluorescence images did not display the expected greater emission from the tumor as compared to the normal mucosa, most probably due to increased optical absorption and scattering in the tumors. Increased fluorescence intensity in neoplasms was observed however, once fluorescence images were corrected using reflectance images. Tryptophan fluorescence alone may be useful in differentiating normal and cancerous cells, while in tissues its autofluorescence image divided by green reflectance may be useful in displaying neoplasms.

History

Received Jul 13, 2011
Accepted Nov 04, 2011
Revised Oct 06, 2011
Published online Feb 01, 2012
Corrected Apr 17, 2012

Digital Object Identifier

http://dx.doi.org/10.1117/1.JBO.17.1.016003

Citation

Bhaskar Banerjee, Timothy Renkoski, Logan R. Graves, Nathaniel S. Rial, Vassiliki Liana Tsikitis, Valentine Nfonsam, Judith Pugh, Piyush Tiwari, Hemanth Gavini and Urs Utzinger, "Tryptophan autofluorescence imaging of neoplasms of the human colon", J. Biomed. Opt. 17, 016003 (Feb 01, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.016003