Imaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope

Saldua, Meagan A.; Olsovsky, Cory A.; Callaway, Evelyn S.; Chapkin, Robert S.; Maitland, Kristen C.

doi:doi:10.1117/1.JBO.17.1.016006

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Journal of Biomedical Optics / Volume 17 / Issue 1 / Research Papers: Imaging

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Imaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope

J. Biomed. Opt. 17, 016006 (Feb 08, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.016006

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Abstract

References (33)

Meagan A. Saldua, Cory A. Olsovsky, and Kristen C. Maitland

Texas A&M University, Department of Biomedical Engineering, 3120 TAMU, College Station, Texas, 77843-3120

Evelyn S. Callaway and Robert S. Chapkin

Texas A&M University, Program in Integrative Nutrition & Complex Diseases, 2253 TAMU, College Station, Texas 77843-2253

Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue features along the length of the entire excised mouse colon. The beam is scanned at 8,333 lines/sec by a polygon scanning mirror while the specimen is scanned in the orthogonal axis by a motorized translation stage with a maximum speed of 7 mm/sec. A single 1×60 mm² field of view image spanning the length of the mouse colon is acquired in 10 s. Z-projection images generated from axial image stacks allow high resolution imaging of the surface of non-flat specimens. In contrast to the uniform size, shape, and distribution of colon crypts in confocal images of normal colon, confocal images of chronic bowel inflammation exhibit heterogeneous tissue structure with localized severe crypt distortion.

History

Received Aug 15, 2011
Accepted Nov 14, 2011
Revised Nov 10, 2011
Published online Feb 08, 2012

Digital Object Identifier

http://dx.doi.org/10.1117/1.JBO.17.1.016006

Citation

Meagan A. Saldua, Cory A. Olsovsky, Evelyn S. Callaway, Robert S. Chapkin and Kristen C. Maitland, "Imaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope", J. Biomed. Opt. 17, 016006 (Feb 08, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.016006