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Journal of Biomedical Optics / Volume 17 / Issue 1 / Research Papers: Imaging

Establishment of rules for interpreting ultraviolet autofluorescence microscopy images for noninvasive detection of Barrett’s esophagus and dysplasia

J. Biomed. Opt. 17, 016013 (Feb 13, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.016013

Bevin Lin, Dennis L. Matthews, and Stavros G. Demos

University of California, Davis, NSF Center for Biophotonics Science and Technology, 4800 2nd Avenue, Sacramento, California 95817

University of California, Davis, Department of Biomedical Engineering, One Shields Avenue, Davis, California 95616

Shiro Urayama

University of California, Davis Medical Center, Division of Gastroenterology and Hepatology, 4150 V Street, Suite 3500, Sacramento, California 95817

Ramez M. G Saroufeem

University of California, Davis Medical Center, Department of Pathology, 4400 V Street, Sacramento, California 95817

Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94550

The diagnostic potential of autofluorescence (AF) microscopy under ultraviolet (UV) excitation is explored using ex vivo human specimens. The aim is to establish optical patterns (the rules for interpretation) that correspond to normal and abnormal histologies of the esophagus, spanning from early benign modifications (Barrett’s esophagus) to subsequent dysplastic change and progression toward carcinoma. This was achieved by developing an image library categorized by disease progression. We considered morphological changes of disease as they are compared with histological diagnosis of the pathological specimen, as well as control samples of normal esophagus, proximal stomach, and small intestine tissue. Our experimental results indicate that UV AF microscopy could provide real-time histological information for visualizing changes in tissue microstructure that are currently undetectable using conventional endoscopic methods.

© 2012 Society of Photo-Optical Instrumentation Engineers

History
Received Jul 12, 2011
Accepted Nov 30, 2011
Revised Nov 01, 2011
Published online Feb 13, 2012
Digital Object Identifier
http://dx.doi.org/10.1117/1.JBO.17.1.016013
Citation
Bevin Lin, Shiro Urayama, Ramez M. G Saroufeem, Dennis L. Matthews and Stavros G. Demos, "Establishment of rules for interpreting ultraviolet autofluorescence microscopy images for noninvasive detection of Barrett’s esophagus and dysplasia", J. Biomed. Opt. 17, 016013 (Feb 13, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.016013

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