Identifying compositional and structural changes in spongy and subchondral bone from the hip joints of patients with osteoarthritis using Raman spectroscopy

Buchwald, Tomasz; Niciejewski, Krzysztof; Kozielski, Marek; Szybowicz, Mirosław; Siatkowski, Marcin; Krauss, Hanna

doi:doi:10.1117/1.JBO.17.1.017007

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Journal of Biomedical Optics / Volume 17 / Issue 1 / Research Papers: Sensing

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Identifying compositional and structural changes in spongy and subchondral bone from the hip joints of patients with osteoarthritis using Raman spectroscopy

J. Biomed. Opt. 17, 017007 (Jan 31, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.017007

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Abstract

References (58)

Tomasz Buchwald, Marek Kozielski, and Mirosław Szybowicz

Poznan University of Technology, Faculty of Technical Physics, Nieszawska 13a, 60-965 Poznań, Poland

Krzysztof Niciejewski and Hanna Krauss

Poznan University of Medical Sciences, Faculty of Medicine I, Święcickiego 6, 60-784 Poznań, Poland

Marcin Siatkowski

DZNE, German Center for Neurodegenerative Disorders, Gehlsheimer Strasse 20, D-18147 Rostock, Germany

University of Rostock, Institute for Biostatistics and Informatics in Medicine and Ageing Research, Ernst Heydemann-Strasse 8, D-18057 Rostock, Germany

Raman microspectroscopy was used to examine the biochemical composition and molecular structure of extracellular matrix in spongy and subchondral bone collected from patients with clinical and radiological evidence of idiopathic osteoarthritis of the hip and from patients who underwent a femoral neck fracture, as a result of trauma, without previous clinical and radiological evidence of osteoarthritis. The objectives of the study were to determine the levels of mineralization, carbonate accumulation and collagen quality in bone tissue. The subchondral bone from osteoarthritis patients in comparison with control subject is less mineralized due to a decrease in the hydroxyapatite concentration. However, the extent of carbonate accumulation in the apatite crystal lattice increases, most likely due to deficient mineralization. The alpha helix to random coil band area ratio reveals that collagen matrix in subchondral bone is more ordered in osteoarthritis disease. The hydroxyapatite to collagen, carbonate apatite to hydroxyapatite and alpha helix to random coil band area ratios are not significantly changed in the differently loaded sites of femoral head. The significant differences also are not visible in mineral and organic constituents’ content in spongy bone beneath the subchondral bone in osteoarthritis disease.

History

Received Aug 22, 2011
Revised Nov 09, 2011
Published online Jan 31, 2012

Digital Object Identifier

http://dx.doi.org/10.1117/1.JBO.17.1.017007

Citation

Tomasz Buchwald, Krzysztof Niciejewski, Marek Kozielski, Mirosław Szybowicz, Marcin Siatkowski and Hanna Krauss, "Identifying compositional and structural changes in spongy and subchondral bone from the hip joints of patients with osteoarthritis using Raman spectroscopy", J. Biomed. Opt. 17, 017007 (Jan 31, 2012); http://dx.doi.org/10.1117/1.JBO.17.1.017007