Spatially parallel control of DNA reactions in optically manipulated microdroplets

Ogura, Yusuke; Nishimura, Takahiro; Tanida, Jun

doi:doi:10.1117/1.3574171

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Journal of Nanophotonics / Volume 5 / Special Section on Nanoengineering: Fabrication, Properties, Optics, and Devices

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Spatially parallel control of DNA reactions in optically manipulated microdroplets

J. Nanophoton. 5, 051702 (May 05, 2011); http://dx.doi.org/10.1117/1.3574171

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Abstract

References (19)

Yusuke Ogura, Takahiro Nishimura, and Jun Tanida

Osaka University, Graduate School of Information Science and Technology, 1-5 Yamadaoka, Suita, Osaka 565-0871, Japan

In order to show the potential of photonic techniques for realizing nanoscale computing, we examined the operation of DNA reactions by optical manipulation of microdroplets that contain DNA. The processing procedures are reconfigurable owing to flexibility in manipulating the microdroplets. The method is effective in, for example, implementing DNA computations in limited-volumes at multiple positions in parallel, enhancing an operation rate, and decreasing sample consumption, and it can be a promising technique applicable to photonic DNA computing. A reaction scheme using a pair of hairpin DNA and linear DNA was examined to confirm the method. The reaction scheme provides exchange of the sequence of a sticky-end of a DNA conformation, and it is usable for DNA computation. Microdroplets that contain DNA components were contacted to each other to start the reaction. By observing fluorescence intensity, we confirmed the reaction of sequence-change in the optically manipulated microdroplet. The experimental result also showed that different reactions are implemented at separate positions simultaneously.

History

Received Oct 01, 2010
Accepted Mar 09, 2011
Revised Feb 28, 2011
Published online May 05, 2011

Digital Object Identifier

http://dx.doi.org/10.1117/1.3574171

Citation

Yusuke Ogura, Takahiro Nishimura and Jun Tanida, "Spatially parallel control of DNA reactions in optically manipulated microdroplets", J. Nanophoton. 5, 051702 (May 05, 2011); http://dx.doi.org/10.1117/1.3574171