Axial resolution enhancement for light sheet fluorescence microscopy via using the subtraction method

Vannhu Le; Xiaona Wang; Cuifang Kuang; Xu Liu

doi:10.1117/1.OE.57.10.103107

30 October 2018 Axial resolution enhancement for light sheet fluorescence microscopy via using the subtraction method

Vannhu Le, Xiaona Wang, Cuifang Kuang, Xu Liu

Author Affiliations +

Optical Engineering, Vol. 57, Issue 10, 103107 (October 2018). https://doi.org/10.1117/1.OE.57.10.103107

Abstract

Light sheet fluorescence microscopy is an imaging method in which the sample is illuminated from the side by a wide and relatively thin light sheet. This method minimizes the out-of-focus excitation, provides a good balance between spatial resolution and temporal resolution, and mitigates photobleaching and photodamage. Unfortunately, the axial resolution of light sheet fluorescence microscopy is much lower than lateral resolution, which limits the achievement of isotropic imaging. We propose to combine the subtraction method with light sheet fluorescence microscopy to improve the axial resolution. The experimental results demonstrate that the proposed method can produce higher axial resolution than that of conventional light sheet fluorescence microscopy. In addition, a way to reduce negative values in subtraction method is also discussed.

Citation Download Citation

Vannhu Le, Xiaona Wang, Cuifang Kuang, and Xu Liu "Axial resolution enhancement for light sheet fluorescence microscopy via using the subtraction method," Optical Engineering 57(10), 103107 (30 October 2018). https://doi.org/10.1117/1.OE.57.10.103107

Received: 12 June 2018; Accepted: 9 October 2018; Published: 30 October 2018

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