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Vertical differential interference contrast
Opt. Eng. 51, 013204 (Feb 06, 2012); http://dx.doi.org/10.1117/1.OE.51.1.013204
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Add to FacebookWe propose a new phase contrast filtering technique based on a combination of a focused and a defocused point-spread-function. This way, an axial shear is introduced in the imaging system. Compared to conventional differential interference contrast, an isotropic behavior is achieved. The lateral resolution is improved compared to conventional defocusing. Furthermore, the digital combination of multiple images leads to strongly enhanced visualization of small structures. We show simulated results as well as experimental results using a spatial-light modulator-based microscope.
© 2012 Society of Photo-Optical Instrumentation Engineers
History
Received Sep 27, 2011
Accepted Nov 29, 2011
Revised Nov 22, 2011
Published online Feb 06, 2012
Accepted Nov 29, 2011
Revised Nov 22, 2011
Published online Feb 06, 2012
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Michael Warber, Tobias Haist, Malte Hasler and Wolfgang Osten, "Vertical differential interference contrast",
Opt. Eng. 51, 013204 (Feb 06, 2012); http://dx.doi.org/10.1117/1.OE.51.1.013204
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