Femtosecond laser printing of living cells using absorbing film-assisted laser-induced forward transfer

Hopp, Béla; Smausz, Tomi; Szabó, Gábor; Kolozsvári, Lajos; Kafetzopoulos, Dimitris; Fotakis, Costas; Nógrádi, Antal

doi:doi:10.1117/1.OE.51.1.014302

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Optical Engineering / Volume 51 / Issue 1 / Laser Applications

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Femtosecond laser printing of living cells using absorbing film-assisted laser-induced forward transfer

Opt. Eng. 51, 014302 (Jan 31, 2012); http://dx.doi.org/10.1117/1.OE.51.1.014302

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Abstract

References (22)

Citing Articles (3)

Béla Hopp

Hungarian Academy of Sciences

University of Szeged, Research Group on Laser Physics, Szeged, Hungary

Tomi Smausz and Gábor Szabó

University of Szeged, Department of Optics and Quantum Electronics, Szeged, Hungary

Lajos Kolozsvári and Antal Nógrádi

University of Szeged, Department of Ophthalmology, Faculty of Medicine, Szeged, Hungary

Dimitris Kafetzopoulos

Institute of Molecular Biology and Biotechnology, Foundations for Research and Technology-Hellas, (IMBB-FORTH), Crete, Greece

Costas Fotakis

Institute of Electronic Structure and Laser, Foundations for Research and Technology-Hellas, (IESL-FORTH)

University of Crete, Department of Physics, Crete, Greece

The applicability of a femtosecond KrF laser in absorbing film-assisted, laser-induced forward transfer of living cells was studied. The absorbing materials were 50-nm-thick metal films and biomaterials (gelatine, Matrigel, each 50 μm thick, and polyhydroxybutyrate, 2 μm). The used cell types were human neuroblastoma, chronic myeloid leukemia, and osteogenic sarcoma cell lines, and primary astroglial rat cells. Pulses of a 500-fs KrF excimer laser focused onto the absorbing layer in a 250-μm diameter spot with 225 mJ/cm² fluence were used to transfer the cells to the acceptor plate placed at 0.6 mm distance, which was a glass slide either pure or covered with biomaterials. While the low-absorptivity biomaterial absorbing layers proved to be ineffective in transfer of cells, when applied on the surface of acceptor plate, the wet gelatine and Matrigel layers successfully ameliorated the impact of the cells, which otherwise did not survive the arrival onto a hard surface. The best short- and long-term survival rate was between 65% and 70% for neuroblastoma and astroglial cells. The long-term survival of the transferred osteosarcoma cells was low, while the myeloid leukemia cells did not tolerate the procedure under the applied experimental conditions.

History

Received Aug 05, 2011
Revised Oct 21, 2011
Published online Jan 31, 2012

Digital Object Identifier

http://dx.doi.org/10.1117/1.OE.51.1.014302

Citation

Béla Hopp, Tomi Smausz, Gábor Szabó, Lajos Kolozsvári, Dimitris Kafetzopoulos, Costas Fotakis and Antal Nógrádi, "Femtosecond laser printing of living cells using absorbing film-assisted laser-induced forward transfer", Opt. Eng. 51, 014302 (Jan 31, 2012); http://dx.doi.org/10.1117/1.OE.51.1.014302