The integrity of mitochondrial function is fundamental to cell life, therefore, the detection and understanding of changes taking place in the organelle may allow faster diagnosis. It turns out that Raman spectroscopy is able to detect changes related to mitochondrial dysfunction much faster by tracking changes in redox state of cytochrome. Our research was focused on selecting the appropriate conditions for the use of MitoBADY (2220 cm-1), both in living and fixed endothelial cells. Interestingly, in our research we were able to observe, Raman signature of life band (1604 cm-1) which also characterizes the functioning of mitochondria. The relationship between the 1604 cm-1 and 2220 cm-1 bands also suggests that the reporter can also be an indicator of mitochondrial activity.
Acknowledgement:
This work was supported by the National Science Centre, Poland, OPUS grant (UMO-2018/29/B/ST4/00335 to MB).
Endothelial dysfunction contributes to the pathophysiology of multiple civilization diseases. Biochemical changes of the dysfunctional endothelium are linked to cellular organelles, including mitochondria, nucleus or lysosomes, so organelle-specific insight is needed for better understanding of endothelial pathobiology. Raman imaging, combining chemical specificity with microscopic resolution, is able to detect biochemical changes at subcellular level. However, the detection of spectroscopic markers associated with specific cell organelles, while desirable, cannot easily be achieved by Raman imaging without labeling. Hence, our work focused on development of Raman reporters that opens a new possibilities in subcellular investigation using labeled Raman microscopy with improved specificity.
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