Dr. Brenda F. Narice Profile

Dr. Brenda F. Narice

at Univ of Sheffield

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Publications (2)

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Proceedings Article | 26 February 2019 Presentation + Paper

In vitro characterization of collagen organization in the human cervix using polarization-sensitive optical coherence tomography (PS-OCT)

Wei Li, Brenda Narice, Dilichukwu Anumba, Stephen Matcher

Proceedings Volume 10856, 1085603 (2019) https://doi.org/10.1117/12.2509547

KEYWORDS: Collagen, Birefringence, Cervix, Optical coherence tomography, Tissues, In vitro testing, In vivo imaging, Polarization

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Babies born before they reach full-term are at an increased risk of long-term complications. Currently, there are no accurate ways to predict when preterm birth (PTB) will occur. The cervix, which plays an essential role in maintaining a pregnancy to term, has to remain closed throughout gestation. However, for birth to occur, it has to shorten, soften and dilate. This crucial remodeling process appears to be linked to variations in the arrangement of collagen. Previous in vitro work using X-ray diffraction suggests that collagen fibers exhibit a preferential orientation in the non-gravid cervix: adjacent to the endocervical canal and in the outermost areas, fibers are mainly arranged in a longitudinal fashion whereas in the middle area they are circumferentially organized. We proposed using a potentially non-invasive imaging technique, polarization-sensitive optical coherence tomography (PS-OCT), to detect the changes in the collagen arrangement of human non-gravid cervix (n=10). Qualitatively, we found that PS-OCT is capable of discriminating between the three cervical regions. Quantitatively, the apparent birefringence of these areas is significantly different across all samples (p<0.05). As expected, apparent birefringence is much lower adjacent to the endocervical canal and in the outermost areas. PS-OCT also seems to be capable of estimating the thickness of the cervical epithelium. Our study, therefore, shows that PS-OCT can assess the microstructure of the human cervical collagen in vitro and holds the potential to help us better understand cervical remodeling prior to birth and develop more timely identification and prevention of PTB pending the development of an in vivo probe.

Proceedings Article | 22 February 2019 Paper

Polarization-sensitive swept-source optical coherence tomography for investigating depth, birefringence, depolarization and orientation of collagen structure of human cervix tissue

Wei Li, Brenda Narice, Dilichukwu Anumba, Stephen Matcher

Proceedings Volume 10867, 108673H (2019) https://doi.org/10.1117/12.2507640

KEYWORDS: Collagen, Birefringence, Cervix, Optical coherence tomography, Tissues, Polarization, Tissue optics, In vivo imaging

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Spontaneous preterm birth (sPTB) is one of the most serious causes of neonatal death. However, sPTB is unpredictable at present due to simplistic research. That cervix remodels progressively through collagen alterations plays an important role during gestation, but the study of cervical collagen structure has been limited by the lack of suitable observational method. Polarization-sensitive optical coherence tomography (PSOCT) is a functional extension of intensity-based OCT, which can noninvasively offer additional information, i.e., the light’s polarization state. Thus, the collagen properties of birefringence and depolarization can be obtained by a PSOCT in vivo. A PSOCT has been developed from our in-house swept-source (SS) OCT. In the PS-SS-OCT, a circularly polarized light is used to interact tissue and the backscattered light which carries sample’s polarization information is detected by two channels for measuring the horizontal and vertical polarization state respectively. Several human cervix tissues have been investigated by the PS-SS-OCT in vitro. The birefringence and depolarization information of cervical collagen can be obtained by processing the intensity and phase value of the two channels. Besides the birefringence and depolarization information, a conical beam scan strategy has been applied for exploring orientation of the collagen structure of human cervix. In the conical scan, the illumination beam streams into sample at a 45° of incidence angle, and the sample is imaged by acquiring successive B-scan over sample-rotation spans of 0-360°. Since probe of PSOCT can be easily integrated into a catheter or a hand-held probe, PSSS-OCT with a conical beam scan is an excellent candidate to identify cervical structure in clinical practice.

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