The qualitative and quantitative assessment of amino acids holds immense significance in the realm of life sciences. It assumes a pivotal role in comprehending intricate biological processes and, importantly, in the early detection of potential disease developments, as amino acids serve as precursors for numerous biomarkers. Our amino acid profiling method offers remarkable versatility in this context, positioning it to scrutinize amino acid profiles in body fluids. Our study introduces an innovative approach to simultaneously detect amino acids through high-performance liquid chromatography combined with laser-stimulated fluorescence, displaying a picomolar detection limit. By harnessing the power of laser-stimulated fluorescence detection, our approach offers a novel pathway to enhance the understanding of amino acids' roles in health and disease.
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