Collagenous colitis is a kind of microscopic colitis. It is characterized by chronic watery diarrhea
and abdominal pain. The etiology is still unknown. So far, for the diagnose a histological
evaluation was necessary with the presence of thickened subepithelial collagneous bands in the
lamina propria. A new developed endoscope with a confocal laser allows analysing cellular and
subcellular details of the mucosal layer at high resolution in vivo. In this case report we describe
for the first time to diagnose collagenous colitis during ongoing colonoscopy by using this
confocal endomicroscopy. In a 67 year old female patient with typical symptoms the
characteristic histological changes could be identified in the endomicroscopic view. Biopsies
could be targeted to affected areas and endomicroscopic prediction of the presence of collagenous
bands could be confirmed in all targeted biopsies. First endomicroscopic experience in
microscopic colitis could be confirmed in four additional patients. Future prospective studies are
warranted to further evaluate these initial findings. However, collagenous colitis is frequently
missed and endomicroscopy seems to be the ideal tool for accurate diagnosing collagenous colitis
during ongoing endoscopy.
Martin Goetz, Sebastian Gregor, Christian Fottner, Jose Garcia-Lazaro, Esther Schirrmacher, Oliver Kempski, Peter Bartenstein, Mathias Weber, Stefan Biesterfeld, Peter Galle, Markus Neurath, Ralf Kiesslich
We evaluated a newly developed miniaturized confocal laser microscopy probe for real-time in vivo
molecular and morphological imaging of normal, inflammatory, and malignant tissue in rodents. In the rigid mini-microscopy probe (diameter 7 mm), a single line laser delivers an excitation wavelength
of 488 nm. Optical slice thickness is 7 μm, lateral resolution 0.7 μm. The range of the z-axis is 0 - 250 μm below the
tissue surface. Organ systems were examined in vivo in rodent models of human diseases. FITC-labeled Lycopersion
esculentum lectin was injected or selected cell populations stained for molecular targeting. Morphological imaging
was performed using fluorescein sodium, FITC-labeled dextran, and/or acriflavine hydrochloride. Cellular and subcellular details could be readily visualised in vivo at high resolution. Tissue characteristics
of different organs were rendered at real time. Selective blood cell staining allowed observation of blood flow and
cell migration. Inflammatory diseases such as hepatitis were diagnosed, and tumors were characterized under
microscopic control in vivo. Confocal mini-microscopy allows real time in vivo molecular and morphological histologic imaging at
high resolution of normal and diseased tissue. Since confocal microscopy is applicable to humans, this technology
will have a high impact on different faculties in medicine.
Conference Committee Involvement (14)
Endoscopic Microscopy XIV
2 February 2019 | San Francisco, California, United States
Endoscopic Microscopy XIII
27 January 2018 | San Francisco, California, United States
Endoscopic Microscopy XII
29 January 2017 | San Francisco, California, United States
Endoscopic Microscopy XI
14 February 2016 | San Francisco, California, United States
Endoscopic Microscopy X
8 February 2015 | San Francisco, California, United States
Endoscopic Microscopy IX
2 February 2014 | San Francisco, California, United States
Endoscopic Microscopy VIII
3 February 2013 | San Francisco, California, United States
Endoscopic Microscopy VII
22 January 2012 | San Francisco, California, United States
Endoscopic Microscopy VI
23 January 2011 | San Francisco, California, United States
Endoscopic Microscopy V
25 January 2010 | San Francisco, California, United States
Endoscopic Microscopy IV
25 January 2009 | San Jose, California, United States
Endoscopic Microscopy III
20 January 2008 | San Jose, California, United States
Endoscopic Microscopy II
21 January 2007 | San Jose, California, United States
Endoscopic Microscopy
22 January 2006 | San Jose, California, United States
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.