In understanding the relationship between neuronal structure and functionality, we leverage calcium/voltage imaging to record the propagation of neuronal signals in vivo. To aptly capture these functional dynamics, we present 2-photon Line Excitation Array Detection (2p-LEAD) microscopy. Through shaping the 1035nm excitation beam into a line, 2p-LEAD can operate at 3000 FPS, using a linear array of PMT’s to collect the resulting fluorescence. With the replacement of the scanning mirror for an acousto-optic deflector, 2p-LEAD holds the potential of achieving kilohertz volumetric imaging over the visual cortex of the mice brain.

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Daniel Wu, Adela Ben-Yakar, Samuel Murphy, and Nicholas Watson, "Two-photon line excitation array detection (2p-LEAD) microscopy for monitoring in vivo neuronal activity," Proc. SPIE PC12853, High-Speed Biomedical Imaging and Spectroscopy IX, PC128530A (Presented at SPIE BiOS: January 27, 2024; Published: 13 March 2024); https://doi.org/10.1117/12.3002894.19e6f8f4-5526-ee11-a999-00505691c5e1.