Non-invasive, structural and functional imaging of in vivo zebrafish in various development stages using Jones matrix optical coherence tomography

Antonia Lichtenegger; Pradipta Mukherjee; Junya Tamaoki; Lida Zhu; Lixuan Bian; Ibrahim Abd El-Sadek; Shuichi Makita; Konrad Leskovar; Makoto Kobayashi; Bernhard Baumann; Yoshiaki Yasuno

doi:10.1117/12.2606979

2 March 2022 Non-invasive, structural and functional imaging of in vivo zebrafish in various development stages using Jones matrix optical coherence tomography

Antonia Lichtenegger, Pradipta Mukherjee, Junya Tamaoki, Lida Zhu, Lixuan Bian, Ibrahim Abd El-Sadek, Shuichi Makita, Konrad Leskovar, Makoto Kobayashi, Bernhard Baumann, Yoshiaki Yasuno

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Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720P (2022) https://doi.org/10.1117/12.2606979
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

A polarization sensitive Jones matrix optical coherence tomography (JM-OCT) prototype was utilized to investigate in vivo wildtype zebrafish in different development stages. JM-OCT is a functional extension of conventional intensity-based OCT to visualize polarization properties of samples. We demonstrated simultaneous structural and functional imaging of in vivo wildtype zebrafish at the age of eight-days, one and two-months post-fertilization. The scattering and polarization characteristics of the anatomical features, such as the eye, and the muscles, were investigated and quantified non-invasively and in three-dimensions. Furthermore, the setup was able to visualize dynamic processes like the blood flow in a label-free way.

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Antonia Lichtenegger, Pradipta Mukherjee, Junya Tamaoki, Lida Zhu, Lixuan Bian, Ibrahim Abd El-Sadek, Shuichi Makita, Konrad Leskovar, Makoto Kobayashi, Bernhard Baumann, and Yoshiaki Yasuno "Non-invasive, structural and functional imaging of in vivo zebrafish in various development stages using Jones matrix optical coherence tomography", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720P (2 March 2022); https://doi.org/10.1117/12.2606979

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KEYWORDS

Optical coherence tomography

In vivo imaging

Functional imaging

Polarization

3D image processing

Birefringence

Eye

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