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Simultaneous multi-channels offset imaging provides isotropic images of retinal microstructures free of directionality artifacts and enables phase imaging in the living eye with enhanced visualization contrast. Phase imaging is widely used in microscopy and biomedical imaging to reveal structures not visible in standard imaging due to their low scattering properties. We introduce the technique in high-resolution retinal imaging to visualize microstructures in the living eye with enhanced contrast, that were not visible with other modalities, and without adding contrast agents. Phase imaging has the potential to be quantitative, may have diagnostic value for retinal diseases, and may enable monitoring treatment.
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Mircea Mujat, Ankit Patel, Nicusor V. Iftimia, "Phase imaging of retinal microstructures with multi-channel AOSLO," Proc. SPIE PC12360, Ophthalmic Technologies XXXIII, PC123600W (17 March 2023); https://doi.org/10.1117/12.2650877