Spatiotemporal optogenetic control of cardiomyocyte excitability using a microLED array

Sebastian Junge; Masa Al Masri; Ricci Signorini; Monika Szepes; Jan Guelink; Heiko Brüning; Ina Gruh; Maria Leilani Torres-Mapa; Alexander Heisterkamp

doi:10.1117/12.2662749

17 March 2023 Spatiotemporal optogenetic control of cardiomyocyte excitability using a microLED array

Sebastian Junge, Masa Al Masri, Ricci Signorini, Monika Szepes, Jan Guelink, Heiko Brüning, Ina Gruh, Maria Leilani Torres-Mapa, Alexander Heisterkamp

Author Affiliations +

Proceedings Volume PC12366, Optogenetics and Optical Manipulation 2023; PC1236606 (2023) https://doi.org/10.1117/12.2662749
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Optogenetics has become a ubiquitous tool for studying and controlling the electrical activity in cardiac cells. In this work, we demonstrate optogenetics stimulation using a microLED array. A microLED array with 256 individually addressable switchable pixels (Qubedot GmbH) is integrated in a widefield imaging setup. Localized and patterned stimulation are both demonstrated showing that intensity is sufficient to pace cardiac bodies and bioartificial cardiac tissues. Dynamic control of each individual microLED pixel permits wave steering of calcium waves in a cardiomyocyte syncytium. Our system provides a compact and easy to use platform for optogenetic stimulation of various cardiac models.

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Citation Download Citation

Sebastian Junge, Masa Al Masri, Ricci Signorini, Monika Szepes, Jan Guelink, Heiko Brüning, Ina Gruh, Maria Leilani Torres-Mapa, and Alexander Heisterkamp "Spatiotemporal optogenetic control of cardiomyocyte excitability using a microLED array", Proc. SPIE PC12366, Optogenetics and Optical Manipulation 2023, PC1236606 (17 March 2023); https://doi.org/10.1117/12.2662749

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