Protein-specific infrared spectroscopic imaging at sub-micrometer resolution with fluorescently guided optical photothermal infrared microscopy (Conference Presentation)

Craig Prater; Yeran Bei; Ferenc Borondics; Oxana l. Klementieva

doi:10.1117/12.2649925

15 March 2023 Protein-specific infrared spectroscopic imaging at sub-micrometer resolution with fluorescently guided optical photothermal infrared microscopy (Conference Presentation)

Craig Prater, Yeran Bei, Ferenc Borondics, Oxana l. Klementieva

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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920O (2023) https://doi.org/10.1117/12.2649925
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We demonstrated the potential of applying infrared spectroscopy for neuroscience by increasing both the spatial resolution and the specificity of chemical structure analysis in tissues and cells, thus overcoming the current limitations of conventional fluorescence microscopy and infrared microspectroscopy. Specifically, we used a novel instrumental setup that combines optical photothermal infrared spectroscopy (OPTIR) with an epifluorescent imaging module (EF) to allow IR spectroscopic analysis of fluorescently microglia cells surrounding amyloid plaques in comparison to microglial cells in amyloid-free tissue. We have demonstrated the capability of EF-OPTIR microscopy to locate specific cells and amyloid proteins and assess amyloid structures with sub-micron spatial resolution.

Conference Presentation

Citation Download Citation

Craig Prater, Yeran Bei, Ferenc Borondics, and Oxana l. Klementieva "Protein-specific infrared spectroscopic imaging at sub-micrometer resolution with fluorescently guided optical photothermal infrared microscopy (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920O (15 March 2023); https://doi.org/10.1117/12.2649925

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KEYWORDS

Infrared spectroscopy

Infrared microscopy

Imaging spectroscopy

Infrared radiation

Chemical analysis

Infrared imaging

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