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15 March 2023 Diffusion mapping by Fourier-transform fluorescence recovery after photobleaching (FT-FRAP) for phase separation in drug formulations (Conference Presentation)
Ziyi Cao, Dustin M. Harmon, Ruochen Yang, Aleksandr Razumtcev, Minghe Li, Mark S. Carlsen, Andreas C. Geiger, Alex M. Sherman, Nita Takanti, Jiayue Rong, Lynne S. Taylor, Garth J. Simpson
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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC1239214 (2023) https://doi.org/10.1117/12.2648473
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Periodically patterned photobleaching followed by spatial Fourier transform analysis of the recovery is shown to enable mapping of molecular diffusivity within spatially heterogeneous media. Image segmentation prior to Fourier transform fluorescence recovery after photobleaching (FT-FRAP) enabled quantitatively evaluating diffusion of macromolecules in spatially and chemically complex media. Notably,multi-harmonic analysis by FT-FRAP was able to definitively discriminate and quantify the roles of internal diffusion and exchange to higher mobility interfacial layers in modeling the recovery kinetics within thin amorphous/amorphous phase separated domains, with interfacial diffusion playing a critical role in recovery.
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Ziyi Cao, Dustin M. Harmon, Ruochen Yang, Aleksandr Razumtcev, Minghe Li, Mark S. Carlsen, Andreas C. Geiger, Alex M. Sherman, Nita Takanti, Jiayue Rong, Lynne S. Taylor, and Garth J. Simpson "Diffusion mapping by Fourier-transform fluorescence recovery after photobleaching (FT-FRAP) for phase separation in drug formulations (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC1239214 (15 March 2023); https://doi.org/10.1117/12.2648473
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KEYWORDS
Diffusion
Luminescence
Fourier transforms
Image segmentation
Signal to noise ratio
Image enhancement
Mathematics
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