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Field-enhanced biosensing has attracted considerable attention due to its capability of highly sensitive biomolecular detection and analysis. Here, we demonstrate a variety of nanogap-enhanced spectroscopies (fluorescence, Raman, infrared absorption) of biomolecular complexes using plasmonic and dielectric nanostructures. Plasmonic nanogaps between gold nanoparticles and gold nanoholes enabled us to perform plasmonic digital biosensing with the limit of detection (LOD) down to sub-fM level. All-dielectric nanogap antennas also allowed us for highly sensitive fluorescence immunosensing.
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