We tested whether near-infrared time-resolved spectroscopy (NIRTRS) is relevant to quantifying oxygenation in human skeletal muscles and identifying brown adipose tissue. Nine healthy men completed the 8-h constant sitting experiment with or without a lower-pressure elastic garment. Prolonged sitting for 8 h induced muscle deoxygenation in lower limb muscles. Conversely, application of the garment successfully prevented the pathophysiological deterioration associated with prolonged sitting. Then, NIRTRS parameters were compared to those determined by 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) with computed tomography (CT) (n=30). The [total-Hb] under thermoneutral conditions are positively correlated with a cold-induced PET parameter (r =0.73, p < 0.05. We conclude that NIRTRS successfully detected deteriorated muscle oxygenation during prolonged sitting and that NIRTRS-determined [total-Hb] in the supraclavicular region is a useful parameter for evaluating brown adipose tissue characteristics in a simple, rapid, non-invasive manner.
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