Tissue and cellular imaging enabled by a fiber laser with optimized balanced detector

Maximilian Brinkmann; Maryam Rezaei; Felix Neumann; Ramon Droop; Christoph Engwer; Niklas Lüpken; Sven Dobner; Tim Hellwig

doi:10.1117/12.3001402

13 March 2024 Tissue and cellular imaging enabled by a fiber laser with optimized balanced detector

Maximilian Brinkmann, Maryam Rezaei, Felix Neumann, Ramon Droop, Christoph Engwer, Niklas Lüpken, Sven Dobner, Tim Hellwig

Author Affiliations +

Proceedings Volume PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry; PC128390O (2024) https://doi.org/10.1117/12.3001402
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Hyperspectral Stimulated Raman Imaging (SRS) has shown great promise as a label-free chemical imaging technique in biomedical and medical research. We present recent developments in SRS integrating a compact and portable all-fiber laser with balanced detection into an imaging system, aiming to enhance ease-of-use, specificity, and reliability in acquiring high-speed, multicolor chemical images. The system's adaptability is highlighted by integrating the entire microscopy system into a clinical cart, ensuring clinical compatibility as well as its seamless integration with a Nikon Eclipse Ti widefield microscope, providing a compact and robust extension for varied imaging setups. The system incorporates a balanced detector to enable shot-noise-limited measurements, accommodating over 100mW of Stokes power on the detector.

Conference Presentation

Citation Download Citation

Maximilian Brinkmann, Maryam Rezaei, Felix Neumann, Ramon Droop, Christoph Engwer, Niklas Lüpken, Sven Dobner, and Tim Hellwig "Tissue and cellular imaging enabled by a fiber laser with optimized balanced detector", Proc. SPIE PC12839, Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry, PC128390O (13 March 2024); https://doi.org/10.1117/12.3001402

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