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13 March 2024 Digital holographic microscopy for label-free detection of NETosis
Kerem Delikoyun, Oliver W. Meldrum, Qianyu Chen, Komal Kumar Javarappa, Matthew J. Worley, Wei Liu, Klaus Diepold, Sanjay H. Chotirmall, Oliver Hayden
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Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC128540W (2024) https://doi.org/10.1117/12.2688254
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Digital holographic microscopy (DHM) offers a label-free and high-throughput imaging approach for biomedical applications. In this study, we present a protocol utilizing DHM to quantify the characteristic morphological changes of neutrophils during Neutrophil Extracellular Trap formation (NETosis). Our method provides a non-invasive and cost-effective solution, eliminating the need for time-consuming sample preparation required for traditional blood smears or fluorescence flow cytometry. By harnessing the unique capabilities of DHM, we aim to provide a tool for the understanding of various inflammatory diseases and to support clinical intervention decisions at the point-of-care.
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Kerem Delikoyun, Oliver W. Meldrum, Qianyu Chen, Komal Kumar Javarappa, Matthew J. Worley, Wei Liu, Klaus Diepold, Sanjay H. Chotirmall, and Oliver Hayden "Digital holographic microscopy for label-free detection of NETosis", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540W (13 March 2024); https://doi.org/10.1117/12.2688254
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KEYWORDS
Digital holography
Holography
Microscopy
Microfluidics
Biological imaging
Holograms
Whole blood
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