Optical Diffraction And Spatial Filtering Of Electron Micrographs Of Biological Materials

G. H. Weyer; P. Dunn; J. S. Wiberg; B. J. Thompson

doi:10.1117/12.958873

22 August 1980 Optical Diffraction And Spatial Filtering Of Electron Micrographs Of Biological Materials

G. H. Weyer, P. Dunn, J. S. Wiberg, B. J. Thompson

Author Affiliations +

Proceedings Volume 0232, 1980 Intl Optical Computing Conf II; (1980) https://doi.org/10.1117/12.958873
Event: 1980 Technical Symposium East, 1980, Washington, D.C., United States

Abstract

Optical diffraction and spatial filtering methods have been used to determine the characteristics of periodic structures in many biological materials. The head shell of bacteriophage T4 has been chosen for this study, since aberrations in the assembly of the shell due to mutation or changes in growth conditions lead to the formation of a variety of elongated tubular head forms. The lattice parameters of structures assembled at elevated growth temperatures by normal, wild-type T4 and by a mutant (regA) have been analyzed using optical diffraction patterns obtained from electron micrographs. Spatial filtering procedures have been used for the reconstruction of one-sided images to determinE the characteristics of the head structures assembled under different growth conditions.

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G. H. Weyer, P. Dunn, J. S. Wiberg, and B. J. Thompson "Optical Diffraction And Spatial Filtering Of Electron Micrographs Of Biological Materials", Proc. SPIE 0232, 1980 Intl Optical Computing Conf II, (22 August 1980); https://doi.org/10.1117/12.958873

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