Research on virtual pinhole parameters optimization in laser differential confocal theta microscope

Xiangye Zhao; Yun Wang; Lirong Qiu; Weiqian Zhao; Ke Zhu

doi:10.1117/12.2266831

10 February 2017 Research on virtual pinhole parameters optimization in laser differential confocal theta microscope

Xiangye Zhao, Yun Wang, Lirong Qiu, Weiqian Zhao, Ke Zhu

Proceedings Volume 10250, International Conference on Optical and Photonics Engineering (icOPEN 2016); 102501Y (2017) https://doi.org/10.1117/12.2266831
Event: Fourth International Conference on Optical and Photonics Engineering, 2016, Chengdu, China

Abstract

Differential confocal theta microscope (DCTM) which has high axial resolution, provides a feasible tool for precise measurement. The virtual pinhole detection technology can significantly simplify the optical path alignment, and enhance imaging quality of the system by optimizing virtual pinhole parameters. Based on the imaging principle of DCTM, a new method for automatic adjusting and optimizing virtual pinhole parameters is presented according to the position and size of the imaging spot, which eliminate the influence caused by the deformation and shifting of imaging spot and ensure the axial resolution. The theoretical analyses and experimental results show that optimizing virtual pinhole parameters can guarantee the axial resolution and signal-to-noise ratio as well as effectively improve the imaging quality of DCTM.

Citation Download Citation

Xiangye Zhao, Yun Wang, Lirong Qiu, Weiqian Zhao, and Ke Zhu "Research on virtual pinhole parameters optimization in laser differential confocal theta microscope", Proc. SPIE 10250, International Conference on Optical and Photonics Engineering (icOPEN 2016), 102501Y (10 February 2017); https://doi.org/10.1117/12.2266831

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